Curriculum Vitae

Profile
A young and motivated graduate of the master biomedical science with a specialization infectious disease and immunology with a background in clinical microbiology. My work experience have increase my competence in practical laboratory work, planning and working in a team.
By analyzing results of project and my master thesis I have sharpened my analytical skills which I would like to use by working in an experienced research team. My main skills are my previously mentioned analytic insight, my problem solving approach and flexible working attitude. I can work both independently and in a team.
My main points are my analytic insight, willingness is to learn, problem solving approach, being flexible and working well independently. That is not to say I do not value working in a team. To further develop my skills a now work as an intern until the 10th of august at the national cancer institute under Dr.Yanling Xiao MD. PhD. But I’m already looking for a new challenging project after I am finished with my project at the national cancer institute.


Education
2011-2013 Master: Biomedical science. Specializations infection diseases and immunology at the Vrije Universiteit of Amsterdam.

-2012: literature study Vrije Universiteit Amsterdam
Supervised by: Dr. Ing. S. van Vliet.
Subject: The role of the Plasmacytoid dendritic cells during
HIV infection and the effect of these cells on the immune
system during the infection.
Reference: S.vanvliet@vumc.nl

2007-2011: Life science specialization medical microbiology at Hogeschool Utrecht.

2001-2007: HAVO, Minkema College Woerden.

Work experience
Project at the national cancer institute
10 March 2014 – 10 August 2014 (6 months)

Developing and testing dendritic cells differentiation methods in vitro under supervision of Dr.Yanling Xiao MD. PhD.

Testing phagocytosis activity of macrophages with live cell and fixed cell microscopy. The number of beads taken up will be determined with the aid of the picture of the live cell microscopy and the program ImageJ. During the project I also followed the course “experimental oncology” of the national cancer institute which I completed successfully.

Summary techniques: Live cell microscopy (CCD), fixed cells microscopy, cell culture, Prism
and IPA ingenuity.
Reference: y.xiao@nki.nl

Research internship
January 2013 – June 2013 (6 months)

Research internship at department of auto immunity at Erasmus MC in Rotterdam under supervision of Prof Dr. Hemmo A. Drexhagen and Ing. H.de Wit.

Determining immune activation in blood of pregnant woman with postpartum psychosis. The results were compared with control woman with and without pregnancy. From the blood monocytes were collected with the automacs and the mRNA was isolated for qPCR with microarrays. From the blood PBMCs and T-cells were isolated and labeled with immunohistochemistry. After labeling the immune profile was determined with the aid of a FACS. Afterwards I analyzed the data with flowjo. Statistical calculations were done with SPSS, graphs were made with Prism. From the results I determined that in both healthy postpartum woman and postpartum psychosis patients the immune system is activated but still normally balanced at 1 month postpartum. In both groups Tregs were still activated at 6 months postpartum. So there was no significant difference found between the two groups based on T cells.

Summary techniques: flow cytometry, immunohistochemistry, monocytes isolation, qPCR,
mRNA isolation and reverse transcriptase. Also worked with flowjo,
SPSS and prism.
Reference: h.drexhage@erasmusmc.nl

Research internships
February 2012 – June 2012 (5 months)

Research internship at VU Amsterdam at department of structural biology under supervision of Prof D.Bald.

Testing the effect of pyrazinoic acid nanoparticles on Mycobacterium bovis BCG (M.bovis). First I determined the toxicity of the nanoparticles again human cancer cells. For This I cultured the cells and after several weeks of incubations determined the cell number with MTT. The results showed that at 12.5 µg/ml the nanoparticles were toxic to human cancer cells. Next I tested the effect of the drug loaded nanoparticles in vitro against M.bovis BCG at different Ph. values. This was done because the drug pyrazinoic acid is Ph. sensitive. After culturing M.bovis BCG and the nanoparticles together I plate the bacteria to determine the colony forming units (CFU). However, the results were inconclusive and a longer incubation then four weeks was required.

Summary techniques: Cell culture, bacteria culture and MTT.
Reference: dirk.bald@falw.vu.nl

Research internship
October 2010 – May 2011 (7 months)

Research internship at department R&D of medical microbiology at the Erasmus MC in Rotterdam under supervision of Dr. A. van Der Bij and Dr. W.H.F Goessens.

Determining the prevalence of carbapenem resistant Pseudomonas aeruginosa in Dutch hospitals. Samples from Dutch hospitals were collected and tested for antibiotic resistance. When the results showed a carbapenem resistant Pseudomonas aeruginosa profile the bacteria was tested for the presence of the carbapenem resistance gene VIM and IMP. This was done with PCR and gel-electrophorese. When positive for either of these genes I determined the genotype to establish if the samples were connected. For this I used the multi locus variable number tandem repeat analysis (MLVA). The results show that most of the carbapenem resistant Pseudomonas aeruginosa carried the VIM gene and belonged to the same or related genotype, although the samples were collected in different hospitals. These results show that the presence of carbapenem resistance Pseudomonas aeruginosa is spreading rapidly.

Summary techniques: bacteria culture, DNA isolation, PCR, MLVA.
Published article: Van der Bij AK, Van der Zwan D, Peirano G.
Metallo-b-lactamase-producing Pseudomonas aeruginosa in the Netherlands: the nationwide emergence of a single sequence type. Clin Microbiol Infect. 2012 Sep;18(9):E369-72
Reference: w.goessens@erasmusmc.nl

Analytic microbiologic internship
February 2011 – June 2011 (5 months)

Analytic microbiologic internship at the department MMIZ at the Erasmus MC under supervision of Rene Kuiper

Competencies
• Consider it to be important to make valuable contributions in a research team toward a common goal.
• Analytical insight in both planning and analyzing results.
• Open to new knowledge, approaches and techniques.
• Resourceful and problem solving approach.

Character references
Inge Heins (Inge.heins@slz.nl)
Head of tech/quality department of pathology of the Slotenvaart Hospital

Bas Hulshof (bas.hulshof@gmail.com)
Head teacher Kung Fu Toa in the Netherlands
Director of Basic Solutions (http://www.basic-solutions.nl/)

Hobbies
• Kung Fu
• Reading
• Writing
• Music

Project at the national cancer institute
10 March 2014 – 10 August 2014 (6 months)

Developing and testing dendritic cells differentiation methods in vitro under supervision of Dr.Yanling Xiao MD. PhD.

Testing phagocytosis activity of macrophages with live cell and fixed cell microscopy. The number of beads taken up will be determined with the aid of the picture of the live cell microscopy and the program ImageJ. During the project I also followed the course “experimental oncology” of the national cancer institute which I completed successfully.

Summary techniques: Live cell microscopy (CCD), fixed cells microscopy, cell culture, Prism
and IPA ingenuity.
Reference: y.xiao@nki.nl

Research internship
January 2013 – June 2013 (6 months)

Research internship at department of auto immunity at Erasmus MC in Rotterdam under supervision of Prof Dr. Hemmo A. Drexhagen and Ing. H.de Wit.

Determining immune activation in blood of pregnant woman with postpartum psychosis. The results were compared with control woman with and without pregnancy. From the blood monocytes were collected with the automacs and the mRNA was isolated for qPCR with microarrays. From the blood PBMCs and T-cells were isolated and labeled with immunohistochemistry. After labeling the immune profile was determined with the aid of a FACS. Afterwards I analyzed the data with flowjo. Statistical calculations were done with SPSS, graphs were made with Prism. From the results I determined that in both healthy postpartum woman and postpartum psychosis patients the immune system is activated but still normally balanced at 1 month postpartum. In both groups Tregs were still activated at 6 months postpartum. So there was no significant difference found between the two groups based on T cells.

Summary techniques: flow cytometry, immunohistochemistry, monocytes isolation, qPCR,
mRNA isolation and reverse transcriptase. Also worked with flowjo,
SPSS and prism.
Reference: h.drexhage@erasmusmc.nl

Research internships
February 2012 – June 2012 (5 months)

Research internship at VU Amsterdam at department of structural biology under supervision of Prof D.Bald.

Testing the effect of pyrazinoic acid nanoparticles on Mycobacterium bovis BCG (M.bovis). First I determined the toxicity of the nanoparticles again human cancer cells. For This I cultured the cells and after several weeks of incubations determined the cell number with MTT. The results showed that at 12.5 µg/ml the nanoparticles were toxic to human cancer cells. Next I tested the effect of the drug loaded nanoparticles in vitro against M.bovis BCG at different Ph. values. This was done because the drug pyrazinoic acid is Ph. sensitive. After culturing M.bovis BCG and the nanoparticles together I plate the bacteria to determine the colony forming units (CFU). However, the results were inconclusive and a longer incubation then four weeks was required.

Summary techniques: Cell culture, bacteria culture and MTT.
Reference: dirk.bald@falw.vu.nl

Research internship
October 2010 – May 2011 (7 months)

Research internship at department R&D of medical microbiology at the Erasmus MC in Rotterdam under supervision of Dr. A. van Der Bij and Dr. W.H.F Goessens.

Determining the prevalence of carbapenem resistant Pseudomonas aeruginosa in Dutch hospitals. Samples from Dutch hospitals were collected and tested for antibiotic resistance. When the results showed a carbapenem resistant Pseudomonas aeruginosa profile the bacteria was tested for the presence of the carbapenem resistance gene VIM and IMP. This was done with PCR and gel-electrophorese. When positive for either of these genes I determined the genotype to establish if the samples were connected. For this I used the multi locus variable number tandem repeat analysis (MLVA). The results show that most of the carbapenem resistant Pseudomonas aeruginosa carried the VIM gene and belonged to the same or related genotype, although the samples were collected in different hospitals. These results show that the presence of carbapenem resistance Pseudomonas aeruginosa is spreading rapidly.

Summary techniques: bacteria culture, DNA isolation, PCR, MLVA.
Published article: Van der Bij AK, Van der Zwan D, Peirano G.
Metallo-b-lactamase-producing Pseudomonas aeruginosa in the Netherlands: the nationwide emergence of a single sequence type. Clin Microbiol Infect. 2012 Sep;18(9):E369-72
Reference: w.goessens@erasmusmc.nl

Analytic microbiologic internship
February 2011 – June 2011 (5 months)

Analytic microbiologic internship at the department MMIZ at the Erasmus MC under supervision of Rene Kuiper